Right here, we report a extremely delicate and dependable floor enhanced Raman scattering (SERS)-based immunoassay utilizing bimetallic alloy Ag@Au hole dual-rim nanorings (DRNs) the place two hole nanorings with completely different diameters are concentrically overlapped and linked by skinny steel ligaments, forming round hot-zones within the intra-nanogaps between inside and outer rims. Pt DRNs had been first ready, after which Ag was deposited on the floor of the Pt skeleton, adopted by Au coating, leading to alloy Ag@Au hole DRNs. The chemical stability of Au and excessive optical properties of Ag are included right into a single entity, Ag@Au hole DRNs, enabling sturdy single-particle SERS exercise and biocompatibility by way of floor modification with thiol-containing functionalities. When Ag@Au hole DRNs had been utilized as nanoprobes for detecting human chorionic gonadotropin (HCG) hormone by way of a SERS-based immunoassay, a really low restrict of detection of 10 pM with excessive reliability was achieved, strongly indicating their benefit as ultrasensitive SERS nanoprobes.