The cooperation relation between biomolecules and nanomaterials makes up a fantastic story about nanoscale chiral sensing and separation. Biomolecules are thought of a wonderful chirality ‘donor’ to develop chiral sensors and separation techniques. Nature has endowed biomolecules with mysterious chirality. Numerous nanomaterials with particular physicochemical attributes can understand the transmission and amplification of this charming chirality. We deal with highlighting the benefits of combining biomolecules and nanomaterials in nanoscale chirality. To boost sensors detection sensitivity, novel cooperation approaches between nanomaterials and biomolecules have attracted large consideration. Furthermore, modern biomolecule-based nanocomposites possess an overarching significance in creating chiral separation techniques with improved assay efficiency. This evaluation describes the formation of a community primarily based on nanomaterials and biomolecules primarily together with DNA, proteins, peptides, amino acids, and polysaccharides. We hope this story will report the perpetual relation between biomolecules and nanomaterials in nanoscale chirality.