The design of nanomaterial-based nucleic acid formulations is likely one of the largest endeavours within the search of clinically relevant gene supply techniques. Biopolymers symbolize a promising subclass of gene carriers on account of their physicochemical properties, biodegradability and biocompatibility. By modifying melanin-like polydopamine nanoparticles with poly-L-arginine and poly-L-histidine blends, we obtained a novel catch-and-release gene supply system for environment friendly trafficking of pDNA to human cells. A synergistic interaction of nanoparticle-bound poly-L-arginine and poly-L-histidine was noticed and evaluated on pDNA binding affinity, cell viability, gene launch and transfection. Though the functionalisation with poly-L-arginine was essential for pDNA binding, the ensuing nanocarriers did not launch pDNA intracellularly, leading to restricted protein expression. Nonetheless, optimum pDNA launch was achieved trough the co-formulation with poly-L-histidine, important for pDNA launch. This impact enabled the design of gene supply techniques, which have been akin to Lipofectamine by way of transfection efficacy and the catch-and-release floor modification technique may be translated to different nanocarriers and surfaces.
