The rising prevalence of antibiotic-resistant bacterial infections, significantly methicillin-resistant Staphylococcus aureus (MRSA), presents a major public well being concern. Well timed detection of MRSA is essential to allow immediate medical intervention, restrict its unfold, and cut back antimicrobial resistance. Right here, we introduce a miniaturized nano-sieve gadget that includes a pneumatically-regulated chamber for extremely environment friendly MRSA purification from human plasma samples. Through the use of packed magnetic beads as a filter and leveraging the deformability of the nano-sieve channel, we obtain an on-chip focus issue of 15 folds for MRSA. We built-in this gadget with recombinase polymerase amplification (RPA) and clustered commonly interspaced brief palindromic repeats (CRISPR)-Cas detection system, leading to an on-chip restrict of detection (LOD) of roughly 100 CFU/mL. This developed strategy gives a speedy, exact, and centrifuge-free answer appropriate for point-of-care diagnostics, with the potential to considerably enhance affected person outcomes in resource-limited medical situations.
