Glycoforms (and different post-translational modifications) of in any other case equivalent proteins can point out pathogenesis/illness state and therefore new instruments to detect and sense a protein’s glycosylation standing are important. Antibody-based assays towards particular protein sequences don’t sometimes discriminate between glycoforms. Right here we show a ‘sandwich’ bio-assay strategy, whereby antibodies immobilised onto biolayer interferometry sensors first choose proteins, after which the particular glycoform is recognized utilizing gold nanoparticles functionalised with lectins which give sign enhancement. The nanoparticles considerably improve the sign relative to lectins alone, permitting glycoform particular detection as little as 0.04 μg mL−1 (1.4 nM) in buffer, and crucially there isn’t any want for an enrichment step and all steps will be automated. Proof of idea is demonstrated utilizing prostate particular antigen: a biomarker for prostate most cancers, the place glycoform evaluation may distinguish between cancerous and non-cancerous standing, slightly than solely detecting general protein focus.
